The inflammatory action is described as a chain of events that arise in response to noxious stimuli, infection or trauma. Various botanical species and plant parts comprise a diverse array of polyphenolic non-steroidal phytochemicals that are incorporated as floral pigments for the attraction of insects as their pollination. Various autoimmune disorders are defined by distinct inflammation and associated failure of the repair process. Pro-inflammatory molecules like TNF, certain interleukins, prostaglandins and even pathogenic concentration of nitric oxide are instrumental in raising such response. More interestingly, nitric oxide has been shown to have the ability to stimulate COX-2 showing a potential synergism. Bryophyllum pinnatum commonly known in some parts of Africa as “good luck” or “resurrection plant”, often used as an herbal remedy for human disorders, including: hypertension, diabetes mellitus, rheumatism, joint pains, insect bites, arthritis, bruises, wounds, boils, abscesses, headaches, and body pains. The leaves are also used for inguinal lymphadenitis and ear diseases. Rue contains different active compounds, out of them rutin, a flavonoid, is known to have nitric oxide scavenging activity.
The eukaryotes sterol pathways are extremely conserved and these biosynthetic pathway are very long which includes the synthesis of dolichols, coenzyme Q, heme A, and isoprenylated proteins.14-Demethylase is an essential enzyme of the cytochrome P450 superfamily, which is potential the target of azole antifungals. Predicted results shows that 14-alpha sterol demethylase have molecular weight of 58930.8 Daltons and the theoretical isoelectric point (pI) of 7.64. The negative Grand average of hydropathicity (GRAVY) index of ‐0.125. The Aliphatic index of Aspergillus fumigates 14-alpha sterol demethylase is 89.48. Alpha helix (Hh) accounts 210 amino acids of about 40.08%. The extended strand (Ee) had 91 amino acids accounting 17.37, Beta turn (Tt) made up of 51 amino acids making up 9.73% and random coil (Cc) made up of 172 amino acids accounting 32.82%. The subcellular localization of 14alpha sterol demethylase Cyp51B was predicted to be a Plasma membrane protein.